TLF has been used for tracing wastewater and industrial effluent discharge signatures owing to the strong link to organic matter. As the technology has evolved, TLF has been widely used as a proxy for microbial activity in water and has been associated with E. coli, coliforms and BOD/COD. Proteus is the first company to harness this and go one step further to actually use the relationship to quantify the target parameter in real-time.